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Phlebotomy


Definition

The etymology of the word Phlebotomy comes from Greek word root phleb/o, meaning vein, /tom/ meaning to cut or puncture, and the suffix /-y which means process. Thus phlebotomy simply means "the process of puncturing veins".

Traditionally, phlebotomy referred to the ancient practice of bloodletting, which involved the withdrawal of larger amounts of blood in an attempt to cure illnesses and disease.

In modern medicine, this practice has been largely abandoned but the term phlebotomy is still widely used for the withdrawal and collection of blood from a vein, artery, or the capillary bed into blood specimen collection tubes for laboratory analysis or blood transfusion. Therefore, blood specimen collection is performed by the phlebotomist.

 



Read more: http://wiki.answers.com/Q/What_is_phlebotomy#ixzz1YfJmrutp

Equipment

  • Tray
  • Mediswab
  • Tourniquet
  • Small adhesive dressing.
  • Sharps Container
  • Gloves
  • Isopropyl alcohol 70% solution hand rub solution
  • ‘Vacutainer’ system:
- needle, holder, appropriate evacuated tubes 
- Or Sterile syringe, Sterile needle, Appropriate evacuated tube 

Vautainer needle holder

Vacutainer needles

Vacutainer system

Tubes Guide

Blood draw filling a purple top vacuum tube

Procedure


  1. Assemble equipment

  2. Inform patient of procedure

  3. Select a suitable vein - e.g. the vein in the antecubital fossa or forearm

  4. Palpate the vessel to exclude the possibility that it is an artery

  5. Apply a tourniquet medial to the selected site

  6. Put on gloves

  7. Cleanse skin with an alcohol wipe

  8. Fix the vein by applying pressure to skin over the vein, approximately two inches below venepuncture site

  9. Leaving the coloured shield on the needle, screw it onto the holder

  10. Remove shield and approach the skin, with needle bevel uppermost at an angle of 35~45 degrees

  11. When the needle has penetrated the skin, realign it with the vein and reduce the angle to about 15 degrees

  12. Introduce the tube into the holder, with middle and forefinger supporting flange of the holder, push the tube with the thumb to the end of the holder, puncturing the diaphragm of the stopper.

  13. As soon as blood starts to flow into the tube, remove the tourniquet.

  14. When blood flow ceases, gently disengage tube from the holder - if more samples are required, repeat from stage 12

  15. Tubes with additives should be gently inverted to mix contents - shaking may cause haemolysis.

  16. Always draw samples without additives first (see videos below).

  17. Place a clean swab or piece of cotton wool over the needle as it is gently withdrawn, pressure should be applied to the site until haemostasis occurs, at which time an adhesive dressing is applied. It is not recommended that the patient bend their arm as this increases the intravascular pressure.

  18. Ensure all samples are clearly labelled

  19. Never re-sheath needles as this is the commonest source of needles stick injury.

  20. Ensure all sharps are disposed of safely and examine holder for any contamination, in which case it should be discarded - in normal practice the holder does not come into contact with blood products and is intended for multiple uses. 

https://www.youtube.com/v/Ie_nFCL5Hp0
 
 
https://www.youtube.com/v/_w3-gtzvenk

https://www.youtube.com/v/v5tPISmRVoQ
Venipuncture Order of Draw

Order of draw

 
    
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